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dc.contributor.advisor Zada, Suher Sallam, Mohamed Abdelazim 2013-01-11T13:22:22Z 2013-01-11T16:00:05Z 2012 Fall 2013-01-11
dc.description.abstract Nanotechnology has enthused excessive expectations in recent years, particularly in the biology and biomedical fields. Carbon-coated metallic nanomagnets reveal significant physicochemical properties, which are referred to as superparamagnetism, that when designed appropriately can be utilized to generate novel diagnostic and therapeutic applications for a wide range of biologically hazardous species. Schistosomiasis is a chronic parasitic disease that can infect both humans and animals, particularly cattle. It is caused by a trematode blood fluke of the genus Schistosoma which belong to the schistosomatidae family. At least 200 million individuals are infected in 75 countries while 600 million people are exposed to infection and are potentially victim of this parasite. Schistosomiasis is still recognized as one of the most neglected disease, the current available diagnostic techniques are deficient in accuracy and are incapable of identifying the disease in its early stages. Moreover, they suffer from prolonged examination time. Besides, the poorly available therapeutic drugs started to lose their efficacy and the parasite started to develop resistance against most of them, which create an imperative need for developing novel diagnostic and therapeutic tools. The humoral immune response of the Camelidae is unique since these animals possess functional heavy-chain only antibodies in addition to the classical antibodies. Thanks to recombinant DNA technology, a series of single domain antigen binding entities can be produced against specific schistosomeâ s parasite antigens. These binders possess a number of distinctive biophysical properties that offer particular advantages in various theranostic applications. Finally, nanomagnetism was selected to represent a certain phenomena through which we demonstrated, to our knowledge for the first time, the successful construction of effective and functional bioconjugate system which is appropriate for a plethora of diagnostic and therapeutic applications. The main thrust of this research work was towards the developing of single domain antibodies against Schistosoma mansoni gut specific antigen and execratory secretory antigen. In parallel, state-of-the-art methodologies were implemented for constructing a bioconjugate system composed of sdAb and graphene-coated metallic nanomagnet tagged with a carboxyl terminal. Finally, the well-established metastable technetium-99 (99mTc) is used for labeling the conjugate for studying the possibility of active and magnetic targeting of the nano-structured system i.e. 99mTc-sdAb-Fe@C-MNP conjugates. en
dc.format.extent 135 p. en
dc.format.medium theses en
dc.language.iso en en
dc.rights Author retains all rights with regard to copyright. en
dc.subject Nanoparticles, Bionanotechnology, Single domain antibodies, Heavy chain only antibodies, Nanobodies, 99mTc radiolabeling en
dc.subject Schistosoma mansoni, Diagnosis and Therapeutic en
dc.subject.lcsh Thesis (M.S.)--American University in Cairo en
dc.subject.lcsh Schistosomiasis -- Treatment.
dc.subject.lcsh Schistosomiasis -- Prevention.
dc.subject.lcsh Nanoparticles -- Research.
dc.title Anti-schistosoma single-domain antibody-nanoparticles conjugate: a novel tool for diagnostic and therapeutic applications en
dc.type Text en
dc.subject.discipline Nanotechnology en
dc.rights.access This item is available en
dc.contributor.department American University in Cairo, School of Sciences and Engineering, Nanotechnology Interdisciplinary Program en
dc.description.irb American University in Cairo Institutional Review Board approval is not necessary for this item, since the research is not concerned with living human beings or bodily tissue samples. en
dc.contributor.committeeMember Muyldermans, Serge
dc.contributor.committeeMember Ramadan, Adham

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  • Theses and Dissertations [1841]
    This collection includes theses and dissertations authored by American University in Cairo graduate students.

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