Show simple item record

dc.contributor.advisor El-Dorry, Hamza Ghabriel, Myret Said 2014-01-15T14:08:22Z 2014-01-15T16:00:04Z 2013 fall en 2014-01-15
dc.description.abstract The long term goal of this research proposal is to further understand the transcriptional regulation of the MRP1 promoter in neuroblastoma cells. We are primarily focused on two possible mechanisms that might lead to the regulation of MRP1 expression: epigenetic modifications, in particular, gene promoter methylation and transcription factors namely the regulation by the methyl binding protein (MeCP2). Within the 5’ untranslated region of the MRP1 promoter is a CpG island that has the potential to be methylated, thus contributing to down regulation of MRP1 expression in drug sensitive neuroblastoma cells. Our goal is to determine if methylation status of the promoter region and subsequently the recruitment of MeCP2 that may play a role in the regulation of MRP1 expression. The methylation status of the promoter will be investigated using Methylation specific PCR and Bisulfite sequencing; while MeCP2 binding will be examined using Chromatin Immunoprecipitation.This project addresses a very important question, which is why neuroblastoma patients develop drug resistance. Identifying regulatory mechanisms for MRP1 expression can potentially help us to determine prognostic factors for drug resistance and further down the road lead to the development of better treatment strategies. en
dc.format.extent 85 p. en
dc.format.medium theses en
dc.language.iso en en
dc.rights Author retains all rights with regard to copyright. en
dc.subject Neuroblastoma en
dc.subject Manufacturing resource planning en
dc.subject.lcsh Thesis (M.S.)--American University in Cairo en
dc.subject.lcsh Neuroblastoma.
dc.subject.lcsh Manufacturing resource planning.
dc.title Modulators of MRP1 promoter in Neuroblastoma cell lines en
dc.type Dataset en
dc.type Still Image en
dc.type Text en
dc.subject.discipline Biotechnology en
dc.rights.access This item is available en
dc.contributor.department American University in Cairo. Dept. of Biology en
dc.description.irb American University in Cairo Institutional Review Board approval is not necessary for this item, since the research is not concerned with living human beings or bodily tissue samples. en
dc.contributor.committeeMember Fouad, Walid
dc.contributor.committeeMember Amleh, Asma
dc.contributor.committeeMember El-Naggar, Shahenda
dc.contributor.committeeMember Abu-Aisha, Khaled

Files in this item


This item appears in the following Collection(s)

  • Theses and Dissertations [1841]
    This collection includes theses and dissertations authored by American University in Cairo graduate students.

Show simple item record