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dc.contributor.advisor Madkour, Tarek Khalifa, Salma Fouad 2016-01-24T10:34:40Z 2016-04-23T22:00:17Z Spring 2016 en_US 2016-01-24
dc.description.abstract Polylactic acid (PLA) is a common biopolymer that is widely used as a biomaterial. However, one of the major issues which limits its further application in tissue engineering purposes is its hydrophobic nature and poor cellular interaction. Modification of PLA properties can be achieved by polymer blending techniques. Polymer blending is a simple yet effective method to combine and optimize the physical properties for polymers of interest. In this study, an antibacterial electrospun nanofibrous scaffolds, with diameters around 400–1000 nm, were prepared by physical blending of PLA with a hydrophylic biopolymer, cellulose acetate (CA). In this stage, PLA was used as the main polymer, blended with CA, at two main ratios (9:1 and 7:3 w/w), to achieve desirable properties such as better hydrophilicity, and cellular attachment and proliferation. For preventing clinical infections, an antimicrobial agent, Thymoquinone, TQ was incorporated into the electrospun nanofibers. TQ is the active ingredient of Nigella sativa and it is well known for its antibacterial properties. The potentiality of the prepared scaffolds, regarding being used as an interactive wound dressing, has been investigated including, swelling behavior, WVP and porosity. The release profile of TQ from the prepared scaffolds was also examined at the physiological pH (7.4) and temperature (37 οC). The antimicrobial efficiency of the prepared scaffolds against gram negative and gram positive bacteria were determined by the agar diffusion assay. The interaction between mouse dermal fibroblasts and the prepared scaffolds such as viability, proliferation, and attachment were characterized. TQ-loaded PLA: CA scaffolds showed burst TQ release after 24 h, compared with TQ-loaded PLA scaffolds, followed by a sustained release for 9 successive days. The results also indicated that TQ-loaded PLA: CA nanocomposite scaffolds showed a significant antibacterial activity against both gram positive and gram negative bacteria. Furthermore, the composite TQ-loaded PLA: CA scaffolds enhanced cell viability, attachment and proliferation, as compared to TQ-loaded PLA. The presence of CA in the nanofiberous scaffolds improved its hydrophilicity, water uptake capacity and water vapor permeability. A preliminary in vivo study was also performed on normal full thickness mice skin wound models. The invivo results showed that TQ-loaded PLA: CA (7:3) scaffolds significantly accelerated the wound healing process through the enhancement of angiogenesis, increasing re-epithelialization and controlling formation of granulation tissue. Consequently, our results suggest that TQ-loaded PLA: CA nanocomposite scaffolds is a promising biomaterial for wound dressing applications.   en_US
dc.format.extent 122 p. en_US
dc.format.medium theses en_US
dc.language.iso en en_US
dc.rights Author retains all rights with regard to copyright. en
dc.subject Wound dressing en_US
dc.subject Poly-lactic acid en_US
dc.subject Polymeric nanofibers en_US
dc.subject cellulose acetate en_US
dc.subject.lcsh Thesis (M.S.)--American University in Cairo en_US
dc.title Fabrication and characterization of antibacterial herbal drug loaded polylactic acid/cellulose acetate nanocomposite nanofibers for wound dressing applications en_US
dc.type Text en_US
dc.subject.discipline Chemistry en_US
dc.rights.access This item is restricted for 3 months from the date issued en_US
dc.contributor.department American University in Cairo. Dept. of Chemistry en_US
dc.description.irb American University in Cairo Institutional Review Board approval is not necessary for this item, since the research is not concerned with living human beings or bodily tissue samples. en_US
dc.contributor.committeeMember Madkour, Tarek
dc.contributor.committeeMember El-sherbiny, Ibrahium Mohamed
dc.contributor.committeeMember Abd-ElGafar, Abd-El-Rahman
dc.contributor.committeeMember Sheoib, Tamer
dc.contributor.committeeMember Ramadan, Adham

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  • Theses and Dissertations [1787]
    This collection includes theses and dissertations authored by American University in Cairo graduate students.

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