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dc.contributor.advisor Suher, Zada Mourad, Lobna Mohamed 2017-10-09T08:34:09Z Summer 2017 en_US 2017-10-09
dc.description.abstract Introduction: The prevalence of hepatocellular carcinoma (HCC) in Africa is higher compared to the rest of the world due to the high incidence of chronic infection with hepatitis C virus (HCV). In Egypt, HCV infection is the leading cause for the high HCC incidence, which is usually diagnosed at late stages. Due to the absence of reliable and accurate biomarkers for early detection of liver cancer, circulating microRNAs have recently emerged as great candidates for early diagnosis of HCC. These small non-coding RNA molecules are responsible for regulating gene expression and RNA stability. Therefore, the aim of this study is to investigate the potential of liver-specific circulating microRNAs as an accurate non-invasive diagnostic tool for the early detection of HCV-induced HCC. Methods: Eight main miRNAs (miR-16, miR-34a, miR-122a, miR-125a, miR-139, miR-145, miR-199a, and miR-221) were selected due to their expression patterns in HCC as well as their contribution to the development of hepato-carcinogenesis. A total of 165 patients were enrolled in this study, from which serum samples were collected and categorized into four main patient groups: 42 chronic hepatitis C (CHC) without cirrhosis, 45 CHC with cirrhosis (LC), 38 HCC with HCV patients, and 40 healthy controls. The expression profile of the eight miRNAs was analyzed using TaqMan real-time reverse transcription-polymerase chain reaction. Additionally, the conventional markers for HCC α-fetoprotein (AFP) and des-γ-carboxyprothrombin (DCP) were measured using commercial kits. Results: Serum levels of miRNA-122a, miRNA-125a, miRNA-139, miRNA-145 and miRNA-199a were significantly lower (p<0.01) in HCC than in both CHC and LC groups. On the other hand, no significant difference was shown in the expression of miR-16, miR-34a, and miR-221 between the CHC, LC, and HCC groups. MiR-16, miR-34a, and miR-221 were significantly elevated in the HCC group compared to the control group. MiR-122a showed the highest specificity and sensitivity, followed by miR-125a, which had the second highest specificity, indicating its significance in diagnosis. Conclusions: The results indicated that measurement of serum levels of miR-122a, miR-125a, miR-139, miR-145, and miR-199a can help to differentiate HCC from CHC and LC. Measurement of serum levels of miR-16, miR-34a, and miR-221 were shown to have a prognostic value. Highly significant correlation was established between different miRNAs within the same patient group or between two different groups, indicating a great diagnostic value for the early detection of HCC. MiR-122a had the highest specificity and sensitivity, indicating that serum miR-122a might serve as a novel and potential non-invasive biomarker for HCV-induced HCC. en_US
dc.description.sponsorship This project is a collaboration between Theodor Bilharz Research Institute (TBRI) and the American University in Cairo (AUC) Biotechnology graduate program. The project was funded by both AUC and TBRI. All patient samples were obtained from TBRI. Thanks for Dr. Hoda Abu Taleb for helping with the statistical analysis. en_US
dc.format.extent 115 p. en_US
dc.format.medium theses en_US
dc.language.iso en en_US
dc.rights Author retains all rights with regard to copyright. en
dc.subject HCV en_US
dc.subject MicroRNAs en_US
dc.subject Hepatocellular Carcinoma en_US
dc.subject.lcsh Thesis (Ph.D.)--American University in Cairo en_US
dc.title Expression analysis of liver-specific circulating micrornas in hcv-induced hepatocellular carcinoma in egyptian patients en_US
dc.type Text en_US
dc.subject.discipline Biotechnology en_US
dc.rights.access This item is restricted for 1 year from the date issued en_US
dc.contributor.department American University in Cairo. Dept. of Biology en_US
dc.embargo.lift 2018-10-09T08:34:09Z
dc.description.irb American University in Cairo Institutional Review Board approval has been obtained for this item. en_US
dc.contributor.committeeMember Khalifa, Amani
dc.contributor.committeeMember El-Ahwany, Eman
dc.contributor.committeeMember Moustafa, Ahmed
dc.contributor.committeeMember Zoheiry, Mona
dc.contributor.committeeMember Abdellatif, Ahmed

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  • Theses and Dissertations [1528]
    This collection includes theses and dissertations authored by American University in Cairo graduate students.

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