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dc.contributor.advisor Amleh, Asma Ghouraba, Mennatallah 2018-05-15T07:03:19Z 2020-05-14T22:00:07Z Spring 2018 en_US 2018-05-15
dc.description.abstract Hepatocellular carcinoma ranks as one of the most lethal types of cancer worldwide and in Egypt. Being complex and heterogenous clinically and molecularly, HCC has a very poor prognosis, and it lacks adequate diagnostic and prognostic molecular markers. Cofactor of BRCA1 (COBRA1); also known as NELF-B, is one of the NELF complex components that stalls RNA polymerase II during transcription elongation and regulates several processes in the cell. Previous studies have shown the upregulation of COBRA1 in Hepatocellular carcinoma (HCC) compared to healthy liver tissue. It was also shown to support cell proliferation and migration in early stages of HCC. In our study, we investigated the role of COBRA1 in the epithelial-mesenchymal transition (EMT)- a critical process in cancer metastasis, and progression of HCC. Functional analysis was done by silencing the expression of COBRA1 in an intermediate stage HCC cell line SNU449, followed by examining the effect of the knockdown on some cancer hallmarks. COBRA1 knockdown cells showed decreased cell proliferation, induction of apoptosis, accompanied by a downregulation of Ki67, Survivin that mark for proliferation and anti-apoptosis respectively. Moreover, COBRA1 Knockdown cells showed a decrease in cell migration and invasion, accompanied by decreased expression of TWIST1- one of the key transcription factors that mediate EMT. Our results demonstrated that COBRA1 supports cell proliferation, migration and invasion, and inhibits apoptosis, which implicates it has a critical role in the progression of HCC. en_US
dc.description.sponsorship University Fellowship Laboratory Instruction Graduate Fellowship AUC Research Grant en_US
dc.format.extent 64 p. en_US
dc.format.medium theses en_US
dc.language.iso en en_US
dc.rights Author retains all rights with regard to copyright. en
dc.subject Hepatocellular carcinoma en_US
dc.subject Cancer en_US
dc.subject Oncogene en_US
dc.subject COBRA1 en_US
dc.subject NELF-B en_US
dc.subject Functional analysis en_US
dc.subject Epithelial mesenchymal transition en_US
dc.subject.lcsh Thesis (M.S.)--American University in Cairo en_US
dc.title Cofactor of BRCA1 promotes epithelial-mesenchymal transition and cell proliferation in intermediate stage hepatocellular carcinoma cell line en_US
dc.type Still Image en_US
dc.type Text en_US
dc.subject.discipline Biotechnology en_US
dc.rights.access This item is restricted for 2 years from the date issued en_US
dc.contributor.department American University in Cairo. Dept. of Biology en_US
dc.description.irb American University in Cairo Institutional Review Board approval is not necessary for this item, since the research is not concerned with living human beings or bodily tissue samples. en_US
dc.contributor.committeeMember Abdelnaser, Anwar
dc.contributor.committeeMember Abou Aisha, Khaled
dc.contributor.committeeMember Mamdouh, Wael
dc.contributor.committeeMember Amleh, Asma

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  • Theses and Dissertations [1835]
    This collection includes theses and dissertations authored by American University in Cairo graduate students.

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