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dc.contributor.advisor Mamdouh, Wael Essam, Mohga 2019-05-20T10:30:08Z Spring 2019 en_US 2019-05-20
dc.description.abstract The rate of bacterial resistance is increasing at an enormous rate making many antibiotics ineffective and resulting in oxidative stress. Furthermore, synthetic antibiotics have major side effects that can be life-threatening. Recently, scientific research started shifting its interest from targeting synthetic antibiotic products to natural-based products such as essential oils (EOs) that are eco-friendly, biocompatible, biodegradable, and effective when exposed to pathogens. Several reports about ginger essential oil (GEO) and oregano essential oil (ORGEO) have been recently reported that they possess immense antibacterial and antioxidant properties. The main objective of this research was to efficiently synthesize electrospun nanofibers (NFs) using from polyvinyl alcohol (PVA) and the surfactant tween-80 (T80) to form PVA/T80/GEO, PVA/T80/ORGEO, and PVA/T80/GEO/ORGEO. Furthermore, investigation of their potential antioxidant activities and their antibacterial activities against Escherichia coli (E.coli) and Staphylococcus aureus (S. aureus) was conducted. The fabrication of the nanofibers was accomplished by the electrospinning method by applying high voltages applied to the polymer which forces it to spin-off in the form of spray forming NFs that get collected on the collector plate. Then, characterization of the NFs was successfully performed by the Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy technique. Also, to study the antibacterial effect, E. coli and S. aureus were cultured in Lysogeny Broth liquid media for a day at a temperature of 37 °C. After that, the NFs from different nanocomposite were exposed to the two types of bacteria in a three-well plate. Optical density measurements were then taken on hourly basis using a spectrophotometer to analyse the antibacterial effect. In addition, antioxidant activity was tested by adding 2,2-diphenyl-1-picrylhydrazyl (DPPH) to the NFs for 2 hrs and monitoring them using ultraviolet visible spectroscopy (UV-Vis). The results obtained show enhanced antibacterial and antioxidant activities of the nanocomposite NFs obtained from combinations containing either PVA/T80/GEO, PVE/T80/ORGEO, and PVA/T80/GEO/ORGEO. In addition, although GEO and ORGEO have already been well-researched EOs and their antimicrobial and antioxidant properties are vastly known, the nanocomposite NFs proved to be much more efficient as antibacterials and antioxidants than pure EOs to be used efficiently in biomedical applications. en_US
dc.description.sponsorship I would like to start my acknowledgement by thanking my advisor and my mentor, Dr. Wael Mamdouh. Without his continuous assistance, guidance, and understanding, I wouldn’t have gained all the knowledge that I now possess, nor would I have achieved all the work that was accomplished. I’m truly thankful to all the support he always provided me with which helped me accomplish my goals in the best way possible. I would also like to greatly thank my colleagues and friends from the lab research team whom have provided me with assistance whenever needed. Special gratitude goes to Kegere James for helping me with my antibacterial testing and my SEM images which he never refused to assist me with even in his spare time. I would also like to thank Amro Shetta for providing me with several characterization techniques and guidance throughout my research. Also, great appreciation goes to my colleagues Ahmed Emad, Jailan Essam, Omar Zidan, Fatma Nabil, Khadija Sadek, Sara Omar, and Hind Amr for their great support and assistance. Finally, I would like to give all the thanks and love to my mother, Nashwa Hassan, and Father, Essam Hamza, for their continuous and unconditional support, care, and love. Also, deep gratitude goes to my sisters Mahitab, Mayada, Minat-Allah, and my brother Hamza for pushing me to go forward and giving me all the physchological assistance throughout my journey. Truly, I am thankful to all these amazing people for all the help they gave me to reach my goals and to proudly become the person I turned out to be. en_US
dc.format.extent 108p en_US
dc.format.medium theses en_US
dc.language.iso en en_US
dc.rights Author retains all rights with regard to copyright. en
dc.subject Nanotechnology en_US
dc.subject Nanofibers en_US
dc.subject Antibacterial en_US
dc.subject Antioxidant en_US
dc.subject Polyvinyl Alcohol en_US
dc.subject Ginger en_US
dc.subject Oregano en_US
dc.subject.lcsh Thesis (M.S.)--American University in Cairo en_US
dc.title Fabrication of Polyvinyl alcohol / Ginger / Oregano Nanocomposite Electrospun Nanofibers and their Enhanced Antibacterial and Antioxidant Activities en_US
dc.type Text en_US
dc.subject.discipline Nanotechnology en_US
dc.rights.access This item is restricted for 2 years from the date issued en_US
dc.contributor.department American University in Cairo. Dept. of Chemistry en_US
dc.embargo.lift 2021-05-19T10:30:08Z
dc.description.irb American University in Cairo Institutional Review Board approval is not necessary for this item, since the research is not concerned with living human beings or bodily tissue samples. en_US
dc.contributor.committeeMember Tallima, Hatem
dc.contributor.committeeMember Al-Ghobashi, Medhat
dc.contributor.committeeMember El-Sawy, Ehab

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  • Theses and Dissertations [1787]
    This collection includes theses and dissertations authored by American University in Cairo graduate students.

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